A new ultrasound method to analyse what happens in the brain while executing activity is portable, has a better resolution and can be used in movement. SINC news agency's LAURA CHAPARRO reports on how HBP's partnering project FUSIMICE has validated the technique's effectiveness in active and anesthetised rodents.
After more than two decades in operation, a team of European researchers, led by the physicist Mickaël Tanter of INSERM (France), has designed a new brain imaging technique with functional ultrasound images (fUS), and validated its effectiveness with awake and moving mice.
Zsolt Lenkei, co-author of the research and coordinator of the European project FUSIMICE, says the main benefits of the new tool are a greater spatio-temporal resolution, since it receives better brain signals in relation to noise, it is portable and cheaper (both when purchased and for maintainance).
In order to know which brain areas are stimulated when we perform a specific task, neurologists usually use functional magnetic resonance imaging (fMRI). This information is especially useful for surgical intervention, so that the neurosurgeon knows which areas to avoid and which areas to work on.But magnetic resonance machines are heavy, with fixed tubes composed of powerful magnets that can cause claustrophobia in some patients.
"Our image method works in freely-moving awake animals" says Lenkei, who works at the École Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI) in France.
In the research, published in the Ultrasound in Medicine and Biology Journal, the authors used both anesthetized and awake rodents and moving specimens. In the case of sleeping mice and rats, the ultrasound images were obtained non-invasively, through the skull and the skin and without using contrast agents, which are common in other techniques because they improve the visibility of tissues and fluids.
For the awake mice, the scientists had to first perform a surgery in which they fixed a small metal plate on the skull of the mouse. Using Doppler ultrasoundsound waves that capture the blood flow in different brain regions they obtained images of brain function while the animals were freely moving. According to the study, the results were similar in both awake and anesthetised mice.
Models of autism and chronic pain
The authors propose that the technique be used to analyse the effects of certain drugs or study how a disease progresses in the brain. With this potential, they believe that the new images will serve for the progress of neuroscience in preclinical studies, i.e. in laboratory and animal models.
The research is part of the FUSIMICE project, whose final objective is to find out how brain connectivity maps are modified with certain neurological diseases. In the next few months, scientists will study two models of mouse autism and a chronic pain model using this new tool and the traditional functional magnetic resonance to compare the results.
The project, coordinated by the École Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France) is also integrated by the National Institute of Health and Medical Research (France), the Institute of Experimental Medicine of the Hungarian Academy of Sciences and the University of Antwerp (Belgium).
FUSIMICE is a Partnering Project in the Human Brain Project, one of the Emblematic Research Initiatives of Future and Emerging Technologies (FET Flagships) of Horizon 2020 – the financing program framework for research of the European Union.
Elodie Tiran, Jeremy Ferrier, Thomas Deffieux, Jean-Luc Gennisson, Sophie Pezet, Zsolt Lenkei and Michael Tanter. “Transcranial functional ultrasound imaging in freely moving awake mice and anesthetized young rats without contrast agent”, Ultrasound in Medicine and Biology, 43 (8) 2017. DOI: 10.1016/j.ultrasmedbio.2017.03.011.
SINC produces scientific news for the European SCOPE project, coordinated by FECYT and funded by the European Union through Horizon 2020, its funding program. The SCOPE mission is to communicate visionary research results of partnering projects in the framework of the GrapheneFlagship and the Human Brain Project, as well as to enhance the FET Flagships partnering environment in the European Union.